oryzae were 201.33 per plate that wereįound immersed in PDA agar medium with peripheral arrangement. oryzae had a growth rate of 1.82 mm/h, the mycelial width of 6.74 μm with appressed solani which produced 52.74 per cent RLH and 100 oryzae was found less pathogenic with 41.97 per cent relative lesion height (RLH) and 57.5 perĬent tiller infection as compared to representative isolate of R. None of the samples was found to be infected with R. Sheath blight complex only to the extent of 1.5 per cent. oryzae from one sample indicating that R. The isolations attempted from 67 sheath blight infected samples Zones of Haryana, India during August, 2016. Samples showing sheath blight symptoms were collected from different rice cultivars grown in three agro-ecological To determine the extent of distribution of these pathogen species, infected Sheath blight disease of rice is a complex biotic stress, caused by three Rhizoctonia speices namely, Rhizoctonia Sheath blight complex of rice from Haryana © 2015, Koninklijke Nederlandse Planteziektenkundige Vereniging.Ĭharacterization of Rhizoctonia oryzae associated with Reaction of individual isolate to each genotype revealed different levels of virulence pointing towards high evolutionary potential, thus enabling it to adapt to diverse geographical regions. The hierarchical cluster analysis based on different disease variables classified isolates into six main groups. The virulence pattern of 18 isolates representing genetically diverse groups generated by ISSR profile was quantified on seven rice genotypes/cultivars i.e., Tetep, Jasmine 85, Te-Qing, D-256, D-6766, PR108 and PAU201 with different levels of resistance to rice sheath blight. Genetic structure of the isolates resolved using inter simple sequence repeat primers revealed high degree of polymorphism (PIC value ranging from 0.80 to 0.90), clustering the pathogen population into four major groups. oryzae (1.5 %) and mixed infection of these Rhizoctonia sp. Accurate identification of the fungal species using internal transcribed spacer species-specific primers confirmed presence of R. Sixty-four Rhizoctonia isolates collected from diverse rice growing agro-ecological zones of the north Indian state of Punjab were analyzed for species identification, genetic diversity, morphological features and virulence pattern. Rhizoctonia solani Kuhn, a highly variable pathogen, causes sheath blight of rice globally leading to serious yield losses under favourable environmental conditions.
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